human intestinal epithelial caco 2 cells  (ATCC)

Journal: Nutrients

Article Title: A Combination of Plant-Derived Extracts Modulates Nutrient-Responsive Metabolic Signalling in an In Vitro Gut–Liver–Adipose Model

doi: 10.3390/nu18091393

Figure Lengend Snippet: Cell viability assay of Gastrodia elata rhizome extract on intestinal cells (Caco-2). Data are expressed as mean ± SD (%) of 5 independent experiments, each performed in triplicate and normalised to the control (0%) line. p < 0.05 vs. control.

Article Snippet: Human intestinal epithelial Caco-2 cells and enteroendocrine NCI-H716 cells (American Type Culture Collection, ATCC ® , Manassas, VA, USA) were used to create an in vitro intestinal model that mimics the epithelial–enteroendocrine interface, allowing simultaneous evaluation of epithelial transport and GLP-1 secretion.

Techniques: Viability Assay, Control

Journal: Nutrients

Article Title: A Combination of Plant-Derived Extracts Modulates Nutrient-Responsive Metabolic Signalling in an In Vitro Gut–Liver–Adipose Model

doi: 10.3390/nu18091393

Figure Lengend Snippet: Effects of individual extracts on cell viability in Caco-2 cells under dose–response conditions, and effects of their combination under time-dependent conditions (1–6 h). In ( A ), dose–response effects of Paeonia lactiflora root extract; in ( B ), dose–response effects of Morus alba leaf extract; and in ( C ), comparative effects of the combination ( Gastrodia elata + Morus alba + Paeonia lactiflora ) versus individual components. Gastrodia elata concentration (0.1 mg/mL) were based on published dose–response data as described in and reported in . Data are expressed as mean ± SD (%) from 5 independent experiments, each performed in triplicate and normalized to the control (0%). * p < 0.05 vs. control.

Article Snippet: Human intestinal epithelial Caco-2 cells and enteroendocrine NCI-H716 cells (American Type Culture Collection, ATCC ® , Manassas, VA, USA) were used to create an in vitro intestinal model that mimics the epithelial–enteroendocrine interface, allowing simultaneous evaluation of epithelial transport and GLP-1 secretion.

Techniques: Concentration Assay, Control

Journal: Nutrients

Article Title: A Combination of Plant-Derived Extracts Modulates Nutrient-Responsive Metabolic Signalling in an In Vitro Gut–Liver–Adipose Model

doi: 10.3390/nu18091393

Figure Lengend Snippet: Evaluation of intestinal permeability in Caco-2 cells. ( A ) TEER values measured using EVOM3 voltohmmeter over time (1–6 h). ( B – D ) TJ protein levels (Claudin-1, Occludin, and ZO-1, respectively) were quantified by ELISA after 6 h of treatment. ( E ) Absorption rate over time (1–6 h), calculated according to the equation J = Jmax [C]/(Kt + [C]) using fluorescent probe. ( F ) GLP-1 secretion was measured after 6 h by ELISA kit. The botanical extracts used in this study were derived from Morus alba leaves, Paeonia lactiflora roots, and Gastrodia elata rhizomes. Data in panel ( A ) and ( F ) are presented as mean ± SD from five independent experiments each performed in triplicate. Data in panels ( B – E ) are expressed as mean ± SD from 5 independent experiments, each performed in triplicate and normalized to the control (0%).* p < 0.05 vs. control; α p < 0.05 vs. single components.

Article Snippet: Human intestinal epithelial Caco-2 cells and enteroendocrine NCI-H716 cells (American Type Culture Collection, ATCC ® , Manassas, VA, USA) were used to create an in vitro intestinal model that mimics the epithelial–enteroendocrine interface, allowing simultaneous evaluation of epithelial transport and GLP-1 secretion.

Techniques: Permeability, Enzyme-linked Immunosorbent Assay, Derivative Assay, Control

Journal: bioRxiv

Article Title: CDK4/6 inhibitors enhance oxaliplatin efficacy in colorectal cancer with RB-dependent and tumor-selective activity in intestinal model

doi: 10.64898/2026.04.15.718743

Figure Lengend Snippet: (A) Representative fluorescence images of IEC-6 cells treated for 48 h with oxaliplatin (0.6 μM), abemaciclib (300 nM), palbociclib (400 nM), or their combinations. Viable cells were detected by Calcein AM fluorescence (green), and nuclei were counterstained with Hoechst (blue). Scale bar: 1000 μm. (B) Quantification of Calcein AM–positive cells following treatment with oxaliplatin and abemaciclib. (C) Quantification of Calcein AM–positive cells following treatment with oxaliplatin and palbociclib. (D) Quantification of ethidium homodimer–positive cells in IEC-6 cultures treated with oxaliplatin and abemaciclib. (E) Quantification of ethidium homodimer–positive cells in IEC-6 cultures treated with oxaliplatin and palbociclib. (F) A summary table showing the percentage of viable cells in HCT116 (tumor) and IEC-6 (non-tumor) cell lines across treatments. The data are presented as the mean ± SEM of three independent biological replicates. Quantification was performed by counting Calcein AM–positive cells relative to total nuclei. Statistical analysis was performed using one-way ANOVA followed by Šídák’s multiple comparisons test. Significance levels are indicated as follows: *p < 0.05; **p < 0.01; ***p < 0.001.

Article Snippet: The non-tumoral rat intestinal epithelial cell line IEC-6 (ATCC: CRL-1592), kindly donated by Prof. José Garcia Abreu (ICB/UFRJ), was maintained in the same basal medium (DMEM/F-12 supplemented with 10% FBS) with additional insulin supplementation at a final concentration of 0.1 U/mL throughout all experimental procedures, including treatments.

Techniques: Fluorescence